Resources our lab uses for clearing:
NAVBO Workshop 2019 Slides
These slides cover:
Why clearing and light-sheet imaging is useful
Overview of various clearing methods
Troubleshooting tips for iDISCO
Light-sheet imaging pros/cons
Handling light-sheet data
iDISCO+ Protocol
This is the protocol we use for clearing. Additional details can be found here.
Active List of Working Antibodies
This google sheet contains details of all of the working antibodies we have successfully used with iDISCO.